An application of microscopic investigation on extracted premolar with dyschromia, related to the components of endodontic sealer.

The aim of this study was an assessment of the overall optical characteristics changes in volume of the dentin, after the diffusion of the chemical components included in the sealer throughout the endodontic space, in order to demonstrate the discoloration effect of the endodontic sealer on tooth crown of a recently extracted maxillary premolar. Thin sections were studied using the polarized transmitted light microscopy and under a stereomicroscope, operating in transmitted light and reflected light. There were performed serial images of magnitude 40×. Each image was covered by a grid aimed for microscopic sections volumetric measurements. Therefore, all the serial images were merged and resulted a global image of the entire section surface. Thus, we have analyzed a total of three sections of the same tooth. Based on these sets of images, there were made estimations of the areas affected by colorations, determinations that can be extrapolated to total tooth areas, in terms of volumetric color change of dentin. The proportion of low coloristic infiltrated dentin was very close to the deeply impregnated dentin and the overall impregnated dentin covered half of the total dentin analyzed area.